Identification of Leishmania Species Isolated from Human Cutaneous Leishmaniasis Using PCR Method

N Rahbarian, A Mesgarian, M Mahmoudi Rad, H Hajaran, F Shahbazi, Z Mesgarian, N Taghipour

Abstract


Background: To determine the epidemiological status of cutaneous leishmaniasis outbreak, isolation and identification of the agent parasite, Leishmania, using PCR method in Gonbad-e Qabus County, north Iran, during 2006-2007.

Methods: Data were collected on the prevalence of scars and ulcers over a period of 3 months among 6990 inhabitants of five villages around Gonbad-e Qabus County, north Iran, during 2006-2007. Cultured promastigotes were identified using PCR technique. Its1 and its2 of Non Coding Transcribed region at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were separated by electrophoresis in 1.5% agarose gel (200 mA, 140 V), visualized by staining with ethidium bromide, and photographed.

Results: Among 6990 inhabitants of 5 villages, 62.9% were identified as scars and 1.5% as active lesions. Individuals 11 to 20 years were the most highly infected age group. All the parasite isolates were Leishmania major.

Conclusion: Cutaneous leishmaniasis due to L. major is endemic in Gonbad-e Qabus County, north Iran.


Keywords


Cutaneous leishmaniasis, ITS-PCR, Epidemiology, Iran

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